Lab arithmeticNAD+Not an FDA-approved drug
NAD+ 500 mg: dosing evidence and reconstitution math
A 500 mg vial of NAD+ mixed with 2 mL of diluent is 250 mg/mL, so 10 units on a U-100 syringe (0.1 mL) contain 25 mg.
Is there an established dose?
No FDA label or published human trial defines a dose of NAD+, so we do not publish one. Online "protocols" for it are not evidence.
- NAD+ profile: FDA status, research volume and registered trials.
500 mg vial concentration table
Concentration = vial content ÷ diluent volume. A U-100 insulin syringe holds 1 mL in 100 units, so 1 unit = 0.01 mL.
| Diluent | Concentration | Per 10 units (0.1 mL) | Per 1 unit |
|---|---|---|---|
| 2 mL | 250 mg/mL | 25 mg | 2.5 mg |
| 5 mL | 100 mg/mL | 10 mg | 1 mg |
| 10 mL | 50 mg/mL | 5 mg | 500 mcg |
| 20 mL | 25 mg/mL | 2.5 mg | 250 mcg |
Arithmetic only; it assumes the vial contains exactly what the label states. Independent tests often find vials under- or over-filled, which is why a lot's certificate of analysis matters.
Syringe units for a given amount
Units on a U-100 syringe needed to draw each amount from this 500 mg vial, by diluent volume. The amounts are fractions of the vial (1/50, 1/20, 1/10, 1/5), not dose suggestions.
| Amount | 1 mL diluent | 2 mL diluent | 3 mL diluent |
|---|---|---|---|
| 10 mg | 2 units | 4 units | 6 units |
| 25 mg | 5 units | 10 units | 15 units |
| 50 mg | 10 units | 20 units | 30 units |
| 100 mg | 20 units | 40 units | 60 units |
Units = amount ÷ concentration × 100. Draws above 100 units exceed a 1 mL syringe.
What the research covers
A PubMed search for "nicotinamide adenine dinucleotide"[tiab] returned 17,148 records (4,931 from the last five years), 141 indexed as clinical trials when checked on October 2026.
- Editorial: The Versatile Role of Nicotinamide Adenine Dinucleotide in Immunity.
- NAD(+) (nicotinamide adenine dinucleotide, oxidized form).
- PPARγ-Coactivator-1α, Nicotinamide Adenine Dinucleotide and Renal Stress Resistance.
- Nicotinamide adenine dinucleotide phosphate oxidase activation and neuronal death after ischemic stroke.
- A Targeting Senescence and Recycling Intracellular Nicotinamide Adenine Dinucleotide Strategy for Attenuation of Senescence-Associated Phenotypes.
ClinicalTrials.gov lists 268 registered studies (search), including:
- Small Sample，Unicentric，Randomized, Controlled Clinical Study of Coenzyme I for Injection in the Treatment of Sudden Sensorineural Hearing Loss
- EFFECT OF CIRCUIT TRAINING ON BONE MINERAL DENSITY IN POST MENOPAUSAL PERIOD
- Tissue-Specific Metabolic Reprogramming in Diabetic Complications
- Clinical Trial of Niagen to Examine Recovery in People With Persistent Cognitive and Physical Symptoms After COVID-19
Storage & handling
Follow the storage statement on the product sheet or certificate of analysis. Lyophilized (freeze-dried) peptides are generally kept cold, dry and away from light; once reconstituted, stability is shorter and depends on the compound and diluent. Bacteriostatic water contains 0.9% benzyl alcohol as a preservative; sterile water does not.
Frequently asked questions
How much bacteriostatic water for a 500 mg NAD+ vial?
The diluent volume only sets the concentration: 1 mL gives 500 mg/mL, 2 mL gives 250 mg/mL and 3 mL gives 167 mg/mL. Smaller volumes are more concentrated, so each syringe unit holds more.
How much NAD+ is in 10 units?
At 2 mL of diluent, 10 units (0.1 mL) of a 500 mg vial contain 25 mg; at 1 mL they contain 50 mg.
What is the dose of NAD+?
No FDA label or published human trial defines a dose of NAD+, so we do not publish one. Online "protocols" for it are not evidence.
Related pages
Peptide calculator BAC water calculator Anti-aging & longevity protocols