The Peptide Directory

Bacteriostatic water calculator

Last updated . Reviewed by The Peptide Directory Editorial Team

To make 5 mg/mL from a 10 mg vial, add 2 mL of bacteriostatic water (10 ÷ 5 = 2).

Also called: bacteriostatic water calculator, how much bac water, peptide water calculator.

Calculator

Result

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Reference chart

Milliliters of bacteriostatic water for a target concentration. Large volumes may not fit the vial.
Vial (mg)1 mg/mL2 mg/mL2.5 mg/mL5 mg/mL10 mg/mL
22 mL1 mL0.8 mL0.4 mL0.2 mL
55 mL2.5 mL2 mL1 mL0.5 mL
1010 mL5 mL4 mL2 mL1 mL
1515 mL7.5 mL6 mL3 mL1.5 mL
2020 mL10 mL8 mL4 mL2 mL
3030 mL15 mL12 mL6 mL3 mL
5050 mL25 mL20 mL10 mL5 mL

Worked example

Example: 10 mg vial, target 5 mg/mL → 10 ÷ 5 = 2 mL of water.

How it works

  1. 1. Vial content

    Note the vial content in mg.

  2. 2. Target concentration

    Choose the concentration you want in mg/mL.

  3. 3. Divide

    Water (mL) = vial mg ÷ target mg/mL.

Frequently asked questions

How much bacteriostatic water do I add to a 10 mg vial?

It depends on the concentration you want: 1 mL gives 10 mg/mL, 2 mL gives 5 mg/mL, 3 mL gives about 3.33 mg/mL.

What is bacteriostatic water?

Sterile water with 0.9% benzyl alcohol, a preservative that inhibits bacterial growth so a multi-use vial can be entered more than once.

Bacteriostatic water vs sterile water?

Sterile water has no preservative and is intended for single use. Bacteriostatic water contains benzyl alcohol and is labeled for multiple withdrawals.

Sources

  1. Reconstitution, units and concentration arithmetic: see our lab-handling guide, The Peptide Directory

Label and trial dose references

For doses as printed in FDA labels or used in trials, see the peptide dose reference and the protocol pages.

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