Peptide Reconstitution Calculator

Last updated October 19, 2026. Reviewed by The Peptide Directory Editorial Team

A 10 mg lyophilized vial reconstituted with 2 mL of diluent has a concentration of 5 mg/mL.

Also called: peptide concentration calculator, BAC water calculator, vial dilution calculator.

Result

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Reference chart

Concentration = vial content ÷ diluent volume. Ignores the small volume added by the solid itself.
Vial (mg)	1 mL	2 mL	2.5 mL	3 mL
2	2 mg/mL	1 mg/mL	0.8 mg/mL	0.67 mg/mL
5	5 mg/mL	2.5 mg/mL	2 mg/mL	1.67 mg/mL
10	10 mg/mL	5 mg/mL	4 mg/mL	3.33 mg/mL
15	15 mg/mL	7.5 mg/mL	6 mg/mL	5 mg/mL
20	20 mg/mL	10 mg/mL	8 mg/mL	6.67 mg/mL
30	30 mg/mL	15 mg/mL	12 mg/mL	10 mg/mL

Example: 5 mg vial + 2.5 mL diluent → 5 ÷ 2.5 = 2 mg/mL (2,000 mcg/mL).

1. Note the vial content
Find the amount of peptide in the vial in mg (check net peptide content on the COA if reported).
2. Note the diluent volume
Record the volume of diluent added, in mL.
3. Divide
Concentration in mg/mL = vial mg ÷ diluent mL. Multiply by 1,000 for mcg/mL.

Divide the vial content in mg by the volume of diluent in mL. A 10 mg vial with 2 mL is 5 mg/mL.

No. Adding more diluent lowers the concentration; the total amount in the vial stays the same.

If a COA reports net peptide content, that figure is the more accurate basis for arithmetic.

No. It is arithmetic for laboratory work and does not recommend amounts for people.

For doses as printed in FDA labels or used in trials, see the peptide dose reference and the protocol pages.

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