Lab arithmeticTB-500 (thymosin beta-4 fragment)Not approved (research only)
TB-500 10 mg: dosing evidence and reconstitution math
A 10 mg vial of TB-500 mixed with 2 mL of diluent is 5 mg/mL, so 10 units on a U-100 syringe (0.1 mL) contain 500 mcg.
Is there an established dose?
No FDA label or published human trial defines a dose of TB-500, so we do not publish one. Online "protocols" for it are not evidence.
- TB-500 (thymosin beta-4 fragment) profile: FDA status, research volume and registered trials.
10 mg vial concentration table
Concentration = vial content ÷ diluent volume. A U-100 insulin syringe holds 1 mL in 100 units, so 1 unit = 0.01 mL.
| Diluent | Concentration | Per 10 units (0.1 mL) | Per 1 unit |
|---|---|---|---|
| 1 mL | 10 mg/mL | 1 mg | 100 mcg |
| 2 mL | 5 mg/mL | 500 mcg | 50 mcg |
| 3 mL | 3.333 mg/mL | 333 mcg | 33.3 mcg |
| 5 mL | 2 mg/mL | 200 mcg | 20 mcg |
Arithmetic only; it assumes the vial contains exactly what the label states. Independent tests often find vials under- or over-filled, which is why a lot's certificate of analysis matters.
Syringe units for a given amount
Units on a U-100 syringe needed to draw each amount from this 10 mg vial, by diluent volume. The amounts are fractions of the vial (1/50, 1/20, 1/10, 1/5), not dose suggestions.
| Amount | 1 mL diluent | 2 mL diluent | 3 mL diluent |
|---|---|---|---|
| 200 mcg | 2 units | 4 units | 6 units |
| 500 mcg | 5 units | 10 units | 15 units |
| 1 mg | 10 units | 20 units | 30 units |
| 2 mg | 20 units | 40 units | 60 units |
Units = amount ÷ concentration × 100. Draws above 100 units exceed a 1 mL syringe.
What the research covers
A PubMed search for "thymosin beta-4"[tiab] returned 602 records (92 from the last five years), 3 indexed as clinical trials when checked on October 2026.
- Thymosin beta 4 as an Alzheimer disease intervention target identified using human brain organoids.
- Recombinant human thymosin beta 4 improves ischemic cardiac dysfunction in mice and patients with acute ST-segment elevation myocardial infarction after reperfusion.
- Thymosin β(4) and β(10) Expression in Human Organs during Development: A Review.
- The beta-thymosin enigma.
- Thymosin beta-4 denotes new directions towards developing prosperous anti-aging regenerative therapies.
ClinicalTrials.gov lists 18 registered studies (search), including:
- A Phase 1 Safety Study of the Intravenous Administration of Thymosin Beta in Healthy Volunteers
- Assessment of the Safety and Efficacy of RGN-259 Ophthalmic Solutions for Dry Eye Syndrome : ARISE-2
- A Phase 2 Study of the Safety and Efficacy of Thymosin Beta 4 for Treating Corneal Wounds
- Assessment of the Safety and Efficacy of 0.1% RGN-259 Ophthalmic Solution for the Treatment of NK: SEER-2
Where research vials are listed
43 suppliers in our directory list TB-500 (A–Z): Amino Amigos, Ascension Peptides, Atomik Labz, Backroad Aminos, BioGenix Peptides, BioLongevity Labs, Biotech Peptides, Bulk Peptide Supply, and 35 more. Across 37 vendors we checked, the median in-stock price was $6.45/mg (range $2.50–$16.50); see TB-500 (thymosin beta-4 fragment) prices per mg. A listing is not a quality endorsement; ask for a lot-specific certificate of analysis.
Storage & handling
Follow the storage statement on the product sheet or certificate of analysis. Lyophilized (freeze-dried) peptides are generally kept cold, dry and away from light; once reconstituted, stability is shorter and depends on the compound and diluent. Bacteriostatic water contains 0.9% benzyl alcohol as a preservative; sterile water does not.
Frequently asked questions
How much bacteriostatic water for a 10 mg TB-500 vial?
The diluent volume only sets the concentration: 1 mL gives 10 mg/mL, 2 mL gives 5 mg/mL and 3 mL gives 3.333 mg/mL. Smaller volumes are more concentrated, so each syringe unit holds more.
How much TB-500 is in 10 units?
At 2 mL of diluent, 10 units (0.1 mL) of a 10 mg vial contain 500 mcg; at 1 mL they contain 1 mg.
What is the dose of TB-500?
No FDA label or published human trial defines a dose of TB-500, so we do not publish one. Online "protocols" for it are not evidence.
Related pages
Peptide calculator BAC water calculator TB-500 (thymosin beta-4 fragment) prices per mg Tissue repair & recovery protocols