Lab arithmeticOxytocinFDA-approved
Oxytocin 5 mg: dosing evidence and reconstitution math
A 5 mg vial of Oxytocin mixed with 2 mL of diluent is 2.5 mg/mL, so 10 units on a U-100 syringe (0.1 mL) contain 250 mcg.
Is there an established dose?
Doses of the FDA-approved product (OXYTOCIN) are set by its FDA label, linked below; research-grade vials are not that product.
- OXYTOCIN on Drugs@FDA (HIKMA FARMACEUTICA)
- Oxytocin profile: FDA status, research volume and registered trials.
5 mg vial concentration table
Concentration = vial content ÷ diluent volume. A U-100 insulin syringe holds 1 mL in 100 units, so 1 unit = 0.01 mL.
| Diluent | Concentration | Per 10 units (0.1 mL) | Per 1 unit |
|---|---|---|---|
| 1 mL | 5 mg/mL | 500 mcg | 50 mcg |
| 2 mL | 2.5 mg/mL | 250 mcg | 25 mcg |
| 3 mL | 1.667 mg/mL | 167 mcg | 16.7 mcg |
| 5 mL | 1 mg/mL | 100 mcg | 10 mcg |
Arithmetic only; it assumes the vial contains exactly what the label states. Independent tests often find vials under- or over-filled, which is why a lot's certificate of analysis matters.
Syringe units for a given amount
Units on a U-100 syringe needed to draw each amount from this 5 mg vial, by diluent volume. The amounts are fractions of the vial (1/50, 1/20, 1/10, 1/5), not dose suggestions.
| Amount | 1 mL diluent | 2 mL diluent | 3 mL diluent |
|---|---|---|---|
| 100 mcg | 2 units | 4 units | 6 units |
| 250 mcg | 5 units | 10 units | 15 units |
| 500 mcg | 10 units | 20 units | 30 units |
| 1 mg | 20 units | 40 units | 60 units |
Units = amount ÷ concentration × 100. Draws above 100 units exceed a 1 mL syringe.
What the research covers
A PubMed search for "oxytocin"[tiab] returned 29,398 records (5,736 from the last five years), 2422 indexed as clinical trials when checked on October 2026.
- The physiology and pharmacology of oxytocin in labor and in the peripartum period.
- Is Oxytocin "Nature's Medicine"?
- Oxytocin signaling regulates maternally directed behavior during early life.
- Maternal plasma levels of oxytocin during breastfeeding-A systematic review.
- Neural circuitry for maternal oxytocin release induced by infant cries.
ClinicalTrials.gov lists 935 registered studies (search), including:
- OTR Tablet 40 mg Fed-state Bioequivalence Study
- Oxytocin Treatment of Alcohol Dependence
- Evaluation of the Effectiveness of a Hypnobreastfeeding Education Program Based on the ınformation-Motivation-Behavioral Skills Model in Primiparous Women
- Carbetocin and Oxytocin in Elective Caesarean Section With High Risk of Postpartum Hemorrhage
Where research vials are listed
24 suppliers in our directory list Oxytocin (A–Z): Ascension Peptides, Atomik Labz, BioGenix Peptides, BioLongevity Labs, Biotech Peptides, Core Peptides, Dynamic Peptide, Eros Peptides, and 16 more. A listing is not a quality endorsement; ask for a lot-specific certificate of analysis.
Storage & handling
Follow the storage statement on the product sheet or certificate of analysis. Lyophilized (freeze-dried) peptides are generally kept cold, dry and away from light; once reconstituted, stability is shorter and depends on the compound and diluent. Bacteriostatic water contains 0.9% benzyl alcohol as a preservative; sterile water does not.
Frequently asked questions
How much bacteriostatic water for a 5 mg Oxytocin vial?
The diluent volume only sets the concentration: 1 mL gives 5 mg/mL, 2 mL gives 2.5 mg/mL and 3 mL gives 1.667 mg/mL. Smaller volumes are more concentrated, so each syringe unit holds more.
How much Oxytocin is in 10 units?
At 2 mL of diluent, 10 units (0.1 mL) of a 5 mg vial contain 250 mcg; at 1 mL they contain 500 mcg.
What is the dose of Oxytocin?
Doses of the FDA-approved product (OXYTOCIN) are set by its FDA label, linked below; research-grade vials are not that product.
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